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Registros recuperados: 97
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PERLE - Programme d'expérimentation et de recherche sur l'huître plate Ostrea edulis - Rapport final des sous-programmes 2 et 3 ArchiMer
Arzul, Isabelle; Baillon, Laury; Boudry, Pierre; Bouget, Jean-francois; Chollet, Bruno; Cochet, Helene; Couraleau, Yann; Dubreuil, Christine; Duchene, Julie; Dutartre, Julie; Garcia, Celine; Heloury, Purotu; Heurtebise, Serge; Huchet, Eve; Hussenot, Mathieu; Langlade, Aime; Ollier, Simon; Omnes, Emmanuelle; Pouvreau, Stephane; Roby, Charlotte; Serpin, Delphine; Viard, Frederique; Wacrenier, Candice; Lapegue, Sylvie.
L’huître plate (Ostrea edulis) est une espèce endémique des côtes européennes, elle est présente depuis des millions d’années. Son aire de répartition est très vaste allant de la Norvège jusqu’au Maroc. Elle est également présente en mer Méditerranée, en mer Noire, mais également au Canada, aux Etats-Unis ou encore en Afrique du Sud, à la suite d'introductions pour des raisons aquacoles. En France, à partir de la fin des années 70, de fortes mortalités ont été constatées sur les bancs naturels et en élevage, faisant rapidement chuter la production de 20 000 tonnes à 2 000 tonnes. Ces mortalités ont été attribuées à deux maladies parasitaires : la marteiliose (due au parasite Marteilia refringens) et la bonamiose (due au parasite Bonamia ostreae). Trente...
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Ano: 2014 URL: http://archimer.ifremer.fr/doc/00247/35815/34328.pdf
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Effects of temperature and salinity on the survival of Bonamia ostreae parasite infecting falt oyster Ostrea edulis in sea water ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting flat oysters Ostrea edulis. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could be demonstrated between the development of the disease and environmental parameters such as temperature or salinity. The parasite life cycle, including its survival outside the host is not completely known. Nevertheless, the infection can be directly transmitted by cohabitation between infected and non infected oysters suggesting that the parasite does not need intermediate host to complete its cycle. In the present study, the impact of temperature and salinity on the survival of purified parasites...
Tipo: Text Palavras-chave: Effects; Temperarature; Salinity; Bonamia ostreae; Parasite; Pathology; Flat oyster; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3375.pdf
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Flow cytometry to measure impact of temperature and salinity on the survival of Bonamia ostreae, parasite infecting flat oyster Ostrea edulis, in seawater ArchiMer
Arzul, Isabelle; Bond, Celine; Gagnaire, Beatrice; Morga, Benjamin; Chollet, Bruno; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster, Ostrea edulis. After its first description in June 1979ı in Brittany, the parasite rapidly spread to all French oyster farming areas and in other European countries through transfers of live molluscs. The parasite has been described in different ecosystems from estuaries to open sea and no clear correlations could he demonstrated between the disease development and environmental parameters like temperature or salinity. The infection can he directly transmitted by cohabitation between infected and non infected oysters. Nevertheless, the parasite life cycle, including its survival outside the host, is not completely...
Tipo: Text Palavras-chave: Impact; Salinity; Temperature; Flow cytometry; Bonamia ostreae; Parasite; Ostrea edulis; Oyster.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/acte-3372.pdf
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Contribution to the understanding of the cycle of the protozoan parasite Marteilia refringens ArchiMer
Arzul, Isabelle; Chollet, Bruno; Boyer, S.; Bonnet, D.; Gaillard, Juliette; Baldi, Yoann; Robert, Maeva; Joly, Jean-pierre; Garcia, Celine; Bouchoucha, Marc.
The paramyxean parasite Marteilia refringens infects several bivalve species including European flat oysters Ostrea edulis and Mediterranean mussels Mytilus galloprovincialis. Sequence polymorphism allowed definition of three parasite types ‘M’, ‘O’ and ‘C’ preferably detected in oysters, mussels and cockles respectively.Transmission of the infection from infected bivalves to copepods Paracartia grani could be experimentally achieved but assays fromcopepods to bivalves failed. In order to contribute to the elucidation of the M. refringens life cycle, the dynamics of the infection was investigated in O. edulis, M. galloprovincialis and zooplankton over one year in Diana lagoon, Corsica (France). Flat oysters appeared non-infected while mussels were infected...
Tipo: Text Palavras-chave: Bivalves; Marteilia refringens; Copepods; Paracartia latisetosa; Zooplankton; Parasite life cycle.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00165/27635/25768.pdf
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Ostrea conchaphila : a natural host of Bonamia ostreae ? ArchiMer
Arzul, Isabelle; Chollet, Bruno; Garcia, Celine; Robert, Maeva; Joly, Jean-pierre; Miossec, Laurence; Berthe, Franck.
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Ano: 2005 URL: http://archimer.ifremer.fr/doc/00448/55998/57502.pdf
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One Perkinsus species may hide another: characterization of Perkinsus species present in clam production areas of France ArchiMer
Arzul, Isabelle; Chollet, Bruno; Michel, Justine; Robert, Maeva; Garcia, Celine; Joly, Jean-pierre; Francois, Cyrille; Miossec, Laurence.
Although clam populations in France are known to be infected with protozoans of the genus Perkinsus, no molecular characterization was previously performed on these parasites. Considering that several members of this genus have been associated with mortalities of molluscs worldwide, a study was undertaken in order to characterize these parasites in France. For that purpose, clams, Ruditapes philippinarum and R. decussatus, collected from different production areas and found to be infected with Perkinsus sp. in thioglycolate culture medium, were selected for PCR-RFLP tests and sequencing. Perkinsus olseni was detected in all the investigated areas and results also suggested the presence of P. chesapeaki in Leucate, a lagoon on the Mediterranean coast and in...
Tipo: Text Palavras-chave: Clam parasites; Perkinsus chesapeaki; Perkinsus olseni; Ruditapes philippinarum; Ruditapes decussatus; Ribosomal RNA; Actin; ISH.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00094/20542/18205.pdf
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Effects of temperature and salinity on the survival of Bonamia ostreae, a parasite infecting flat oysters Ostrea edulis ArchiMer
Arzul, Isabelle; Gagnaire, Beatrice; Bond, Celine; Chollet, Bruno; Morga, Benjamin; Ferrand, Sylvie; Robert, Maeva; Renault, Tristan.
Bonamiosis due to the intrahaemocytic protistan parasite Bonamia ostreae is a European endemic disease affecting the flat oyster Ostrea edulis. The parasite has been described in various ecosystems from estuaries to open sea, but no clear correlation has yet been demonstrated between disease development and environmental parameters. In this study, the effect of temperature and salinity on the survival of purified parasites maintained in vitro in seawater was investigated by flow cytometry. Purified parasites were incubated in various seawater media (artificial seawater, natural seawater, seabed borewater) at various temperatures (4, 15 and 25°C) and subjected to a range of salinities from 5 to 45 g l–1. Parasites were collected after 12, 24 and 48 h of...
Tipo: Text Palavras-chave: Salinity tolerance; Temperature tolerance; Cell viability; In vitro assays; Flow cytometry; Bonamia ostreae.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6443.pdf
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Bilan 2003 du réseau REPAMO - Réseau national de surveillance zoosanitaire des mollusques marins ArchiMer
Arzul, Isabelle; Garcia, Celine; Joly, Jean-pierre; Miossec, Laurence; Chollet, Bruno; Robert, Maeva; Cuvelier, Nicolas; Lefebvre, Alain; Le Gagneur, Eric; Kopp, Joel; Mouillard, Gilbert; Gerla, Daniel; Le Saux, Jean-claude; Raguenes, Pierre; Le Gal, Dominique; Langlade, Aime; Bedier, Edouard; Nourry, Max; Martin, Jean-louis; Fillon, Alain.
The REPAMO network (network ofmollusc pathology), created in 1986, ensures the survey of shellfish health status along French coasts according? to European Directives 91/67/EEC and 95/70/EEC. The aims of the network are 1 - to prevent the introduction and spread of exotic pathogens, 2-to study the means to decrease the impact of pathogens already present and to survey their evolution. In 2003, the REPAMO network focused on the survey oflisted diseases (bonamiosis and marteiliosis) in two areas under agreement process (Banc de Granville and zone X) and on the study of abnormal morlalities. Number of reported abnormal morlalities has increased in 2003. This rise can be partly explained by the recent network restructuring, but also by the summer heatwave and...
Tipo: Text Palavras-chave: Réseau; Surveillance; Pathologie; Mollusques; Coquillages; Etat de santé; Network; Surveillance; Pathology; Molluscs; Shellfish; Health status.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/00086/19699/17340.pdf
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Can the protozoan parasite Bonamia ostreae infect larvae of flat oysters Ostrea edulis? ArchiMer
Arzul, Isabelle; Langlade, Aime; Chollet, Bruno; Robert, Maeva; Ferrand, Sylvie; Omnes, Emmanuelle; Lerond, Sophie; Couraleau, Yann; Joly, Jean-pierre; Francois, Cyrille; Garcia, Celine.
Bonamia ostreae is an intracellular protistan parasite affecting flat oysters Ostrea edulis. It can be detected in juveniles but mortalities mainly affect oysters which are more than 2 years old. The parasite is usually observed inside haemocytes and sometimes free, notably in gill epithelia suggesting a parasite release through this organ. However, the infective form and ways of entry and release remain undetermined. Flat oysters incubate their larvae in their pallial cavity for 8–10 days before releasing them into the water column. Flat oysters in Bay of Quiberon in South Brittany (France) are known to be infected with B. ostreae since 1979 and is the most important area in France for O. edulis spat collection. Flat oysters incubating larvae were sampled...
Tipo: Text Palavras-chave: Bonamia ostreae; Flat oyster; Ostrea edulis; Transmission; Larvae; Parasite life cycle.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00028/13898/12566.pdf
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Molecular characterization of parasites of the genus Perkinsus present in clams from French producing areas ArchiMer
Arzul, Isabelle; Michel, Justine; Chollet, Bruno; Robert, Maeva; Miossec, Laurence; Garcia, Celine; Francois, Cyrille.
Production of clams is the third most important bivalve production in France and was estimated at 3400 metric tons in 2001 among which 60% came from aquaculture and 40% from fisheries. Surveillance of clam diseases mainly relies on histology and thioglycolate culture, two techniques allowing detection of parasites of the genus Perkinsus but not specific enough to determine the parasite species. Based on a previous study aiming at estimating distribution and prevalence of perkinsosis in France, we selected 34 infected clams from 4 different producing areas in order to characterize the parasite species. Molecular characterization was done by PCR-RFLP targeting the ITS region as described by Abollo et al. (2006). 16 PCR products were cloned and 10 to 20...
Tipo: Text Palavras-chave: French producing areas; Clams; Perkinsus olseni; Parasite.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/acte-6269.pdf
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Can Bonamia ostreae infect larvae of flat oysters Ostrea edulis ? ArchiMer
Arzul, Isabelle; Robert, Maeva; Garcia, Celine; Chollet, Bruno; Langlade, Aime.
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Ano: 2009 URL: http://archimer.ifremer.fr/doc/00448/55999/57503.pdf
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Réseau de veille zoosanitaire des mollusques REPAMO - Rapport d'activité pour l'année 1997 ArchiMer
Berthe, Franck; Pichot, Yves; Chollet, Bruno; Thebault, Anne; Kuntz, Gregoire; Tige, Gilbert; Thebault, Anne; Fleury, Pierre-gildas; Hirata, Toshio; Le Coguic, Yvette; Renault, Tristan; Lipart, Cecile; Grizel, Henri; Joly, Jean-pierre; Mazurie, Joseph; Baud, Jean-pierre; Goulletquer, Philippe; Dreno, Jean-paul; Buestel, Dominique.
Le réseau de veille zoosanitaire des mollusques, REPAMO, a été créé en 1992 afin de protéger les espèces d'intérêt économique des maladies susceptibles de les affecter. En effet, de même que les autres productions animales, la conchyliculture est frappée par diverses maladies dont certaines se comportent comme des facteurs limitants de la qualité ou de la quantité des productions. L' histoire de l'ostréiculture française a connu de tristes expériences en la matière, notamment dans les années 70 pour l' huître creuse portugaise, Crassostrea angulata ou encore pour l'huître plate, Ostrea edulis. En fait, nous ne disposons que de très peu de possibilités de protéger les mollusques bivalves vis à vis des maladies infectieuses. D' une part, les traitements,...
Tipo: Text Palavras-chave: Réseau surveillance; Pathologie; Mollusques bivalves; Mortalité.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00098/20901/18521.pdf
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Acclimatation de nouvelles espèces d'huîtres creuse du genre Crassostrea: hybridation et conservatoire de souches - Contrat de Plan Etat Région Poitou-Charentes 1994-1998 - Synthèse finale ArchiMer
Boudry, Pierre; Heurtebise, Serge; Huvet, Arnaud; Chollet, Bruno; Ledu, Christophe; Phelipot, Pascal; Gerard, Andre.
Actions menées de 1994 à 1998 au sein du conservatoire de souches : 1. Résultats des importations, acclimatation et hybridation. a) Rappel des conditions d'introduction de nouvelles souches. L'importation de toute espèce est conditionnée par les résultats d'une enquête préliminaire dont l'objet est de rechercher les exigences écologiques de l'espèce et la situation épidémiologique dans son milieu d'origine. Le travail du LGP et de ses partenaires a été partagé en différentes actions: 1. Recherche de contacts dans divers pays étrangers, en vue de l'importation d'huîtres présentant des caractéristiques intéressantes. 2. Importation : L'importation se fait selon les normes du CIEM (Conseil International pour l'Exploration de la Mer) : contrôles pathologiques...
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Marqueurs moléculaire; Marqueurs populations; Crassostrea.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/00044/15520/12907.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridation et conservatoire de souches. Amélioration de la qualité chez l'huître creuse Crassostrea gigas par sélection de souches performantes - Rapport année 1996 ArchiMer
Boudry, Pierre; Naciri, Yamama; Collet, Bertrand; Cornette, Florence; Heurtebise, Serge; Ledu, Christophe; Chollet, Bruno; Phelipot, Pascal; Gerard, Andre.
Les objectifs pour l'année 1996 étaient les suivants: Poursuivre les premières reproductions des espèces pour lesquelles nous disposons de géniteurs GO. L'objectif est toujours de limiter le stockage d'animaux importés en produisant une descendance simultanément à des témoins C. gigas qui serviront de comparaison. Tenter des hybridations inter-spécifiques des différents taxons présents au sein du conservatoire en fonction des connaissances disponibles dans la littérature sur ce sujet (voir pour revue Gaffney et Allen, 1993). • Importer de nouvelles souches ou espèces. Élargir la gamme des espèces présentes au sein du conservatoire de souches (les capacités d'accueil de la salle de quarantaine étant Limitée).
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea; Sélection génétique; Saccostrea commercialis.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00044/15516/12903.pdf
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Acclimatation de nouvelles espèces d'huîtres creuses du genre Crassostrea: hybridations et conservatoire de souches. Contrat de Plan Etat Région Poitou- Charentes 1994-1998 - Convention 95/RPC-R-57 "Génétique" - Rapport année 1995 ArchiMer
Boudry, Pierre; Naciri, Yamama; Heurtebise, Serge; Delsert, Claude; Ledu, Christophe; Phelipot, Pascal; Chollet, Bruno; Cochennec, Nathalie; Gerard, Andre.
Le laboratoire IFREMER "Génétique Aquaculture et Pathologie" (GAP) (La Tremblade et Bouin) a entrepris des actions de recherche sur ces différents points. L'Unité de Recherche en Génétique, en collaboration avec tous les membres du Réseau Génétique Mollusques (REGEMO), a axé une partie de ses travaux sur l'acclimatation et l'hybridation de différentes espèces du genre Crassostrea. Ces recherches ont débuté avec le soutien du Conseil Général de Charente-Maritime depuis 1992 sur des essais d'acclimatation de C. virginica, l'huître creuse américaine. En prolongement de cette étude, l'URGE a proposé à la région Poitou-Charentes un programme de recherche plus vaste sur l'ensemble des espèces d'huîtres creuses.
Tipo: Text Palavras-chave: Génétique; Acclimatation; Hybridation; Crassostrea virgnica.
Ano: 1995 URL: http://archimer.ifremer.fr/doc/00044/15515/12902.pdf
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New evidence for the involvement of Paracartia grani (Copepoda, Calanoida) in the life cycle of Marteilia refringens (Paramyxea) ArchiMer
Boyer, S.; Chollet, Bruno; Bonnet, D.; Arzul, Isabelle.
The dynamics of the protozoan parasite Marteilia refringens was studied in Thau lagoon, an important French shellfish site, for 1 year in three potential hosts: the Mediterranean mussel Mytilus galloprovincialis (Mytiliidae) the grooved carpet shell Ruditapes decussatus (Veneriidae) and the copepod Paracartia grani (Acartiidae). Parasite DNA was detected by PCR in R. decussatus. In situ hybridisation showed necrotic cells of M. refringens in the digestive epithelia of some R. decussatus suggesting the non-involvement of this species in the parasite life cycle. In contrast, the detection of M. refringens in mussels using PCR appeared bimodal with two peaks in spring and autumn. Histological observations of PCR-positive mussels revealed the presence of...
Tipo: Text Palavras-chave: Marteiliosis; Marteilia refringens; Parasite life cycle; Mytilus galloprovincialis; Ruditapes decussatus; Paracartia grani; PCR; In situ hybridisation.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00157/26795/25532.pdf
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Cosmopolitan Distribution of Endozoicomonas-Like Organisms and Other Intracellular Microcolonies of Bacteria Causing Infection in Marine Mollusks ArchiMer
Cano, Irene; Ryder, David; Webb, Steve C.; Jones, Brian J.; Brosnahan, Cara L.; Carrasco, Noelia; Bodinier, Barbara; Furones, Dolors; Pretto, Tobia; Carella, Francesca; Chollet, Bruno; Arzul, Isabelle; Cheslett, Deborah; Collins, Evelyn; Lohrmann, Karin B.; Valdivia, Ana L.; Ward, Georgia; Carballal, María J.; Villalba, Antonio; Marigómez, Ionan; Mortensen, Stein; Christison, Kevin; Kevin, Wakeman C.; Bustos, Eduardo; Christie, Lyndsay; Green, Matthew; Feist, Stephen W..
Intracellular microcolonies of bacteria (IMC), in some cases developing large extracellular cysts (bacterial aggregates), infecting primarily gill and digestive gland, have been historically reported in a wide diversity of economically important mollusk species worldwide, sometimes associated with severe lesions and mass mortality events. As an effort to characterize those organisms, traditionally named as Rickettsia or Chlamydia-like organisms, 1950 specimens comprising 22 mollusk species were collected over 10 countries and after histology examination, a selection of 99 samples involving 20 species were subjected to 16S rRNA gene amplicon sequencing. Phylogenetic analysis showed Endozoicomonadaceae sequences in all the mollusk species analyzed....
Tipo: Text Palavras-chave: Mollusk; OTU; Endozoicomonas; Chlamydiae; Mycoplasma; Francisella; Lucinoma; Ridgeia.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00658/77032/78322.pdf
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Diagnostic des pathologies rencontrées chez les mollusques bivalves marins ArchiMer
Chollet, Bruno.
L'huître creuse, Crassostrea gigas, est aujourd'hui une des espèces de mollusques bivalves marins la plus élevée dans le monde. Cette espèce a été importée du Japon, dans les années 1960-1970, et a remplacé l'huître portugaise, Crassostrea angulata, espéce décimée à cette époque par deux iridoviroses. Suite à la disparition de l'huître portugaise du littoral français et à l'apparition de protozooses (Marteiliose et Bonamiose) chez l'huître plate, Ostrea edulis, une situation de quasi monoélevage de l'huître japonaise s'est installée en France. De ce fait, les risques sur les plans sanitaire et économique sont loin d'être négligeables pour le milieu ostréicole français. En particulier, l'apparition de maladies chez cette espèce pourrait aboutir à une...
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Ano: 1994 URL: http://archimer.ifremer.fr/doc/00429/54085/55402.pdf
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Bonamia-like parasite found in the Suminoe oyster Crassostea rivularis reared in France ArchiMer
Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea. Thus,...
Tipo: Text Palavras-chave: Bonamia like parasite; Bonamiosis; Simunoe oyster; Crassostrea rivularis.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/publication-2904.pdf
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Bonamia-like parasite found in the Suminoe oyster Crassostrea rivularis reared in France ArchiMer
Cochennec, Nathalie; Renault, Tristan; Boudry, Pierre; Chollet, Bruno; Gerard, Andre.
Considering the economic importance of the Pacific oyster Crassostrea gigas to the French shellfish industry, the appearance of major diseases in this species could cause dramatic decreases in production. Suminoe oysters, of the non-indigenous species Crassostrea rivularis (Gould), were introduced into France to test their ability to adapt to local conditions. These oysters were imported after careful examination, and were maintained in laboratory quarantine. Some mortalities occurred 7 mo after importation. Histological and electron microscope examinations of 9 dead specimens revealed a parasite presumed to be a Bonamia-Like protozoan. This is the first report concerning a parasite of the genus Bonamia in a species belonging to the genus Crassostrea....
Tipo: Text Palavras-chave: Crassostrea rivularis; Suminoe oyster; Bonamiosis; Bonamia-like parasite.
Ano: 1998 URL: https://archimer.ifremer.fr/doc/00446/55795/62652.pdf
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